| 纤维素降解菌的筛选及其降解特性的研究 |
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| 引用本文： | 宋颖琦,刘睿倩,杨谦,宋金柱,杜金哲.纤维素降解菌的筛选及其降解特性的研究[J].哈尔滨工业大学学报,2002,34(2):197-200. |
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| 作者姓名： | 宋颖琦 刘睿倩 杨谦 宋金柱 杜金哲 |
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| 作者单位： | 哈尔滨工业大学,理学院,黑龙江,哈尔滨,150001 |
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| 基金项目： | 20 0 0年市科委科技攻关项目 ( 0 0 12 2 110 2 0 ) |
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| 摘 要： | 在自然环境和秸秆降解物中筛选出3种分解纤维素能力较强的木霉、青霉和曲霉，将3种菌株以不同的组合形式在羧甲基纤维素钠为唯一碳源的产酶培养基中进行产酶特性的研究，计算其对玉米秸秆粉中粗纤维的利用率并观测玉米秸秆粉的降解状态。其中木霉与青霉的混合培养在纤维素筛选培养基上培养24h后即可生长，在发酵培养基中培养72h后达到产酶高峰，酶活可达3．116u，对粗纤维的利用率为68％，远超过青霉和木霉的单独培养；木霉与曲霉二者混合，木霉与青霉、曲霉三者混合培养的产酶效果也要超出青霉和曲霉的单独培养，但与木霉相比无明显差异。实验结果表明优良菌株的混合培养会增加其对纤维素的降解能力，为实际生产中有效的处理农作物秸秆提供了理论依据。
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| 关 键 词： | 纤维素降解菌 筛选 降解特性 发酵培养 木霉 青霉 曲霉 农作物秸秆 废弃物处理 |
| 文章编号： | 0367-6234(2002)02-0197-04 |
| 修稿时间： | 2001年4月19日 |
Study of screening of strains for degrading cellulose and mechanism of degrading ability |
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| SONG Ying-qi,LIU Rui-qian,YANG Qian,SONG Jin-zhu,DU Jin-zhe.Study of screening of strains for degrading cellulose and mechanism of degrading ability[J].Journal of Harbin Institute of Technology,2002,34(2):197-200. |
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| Authors: | SONG Ying-qi LIU Rui-qian YANG Qian SONG Jin-zhu DU Jin-zhe |
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| Abstract: | species of fungi were selected from the natured enviroment and the remains of degraded corn straw .Different combinations of these 3 fungi had different cellulase activities .It was found that the cellulase activity can be obviously increased when Trichoderma.sp was mixed with Penicillium.sp.It can reach the peak after 72 hours culturing,the amount is 3.116u, and it can grow rapidly in solid medium with CMC-Na as the sole carbon source, and the utilization ratio of crude cellulose is 68%.This result exceeds the expression of these 2 fungi than that of their separate growth. The cellulase activity of Trichoderma.sp mixed with Aspergillus.sp or with combination of Aspergillus.sp and Penicillium.sp. can also grow faster than the single culture of the Aspergillus.sp and Penicillium.sp,but it is not significantly different from growth of Trichoderma.sp. It is proved that when bombined the strains can increase the degrading ability,offer a new way to deal with the crops straw. |
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| Keywords: | degrading cellulose activity of cellulase mixed culture utilization ratio of crude cellulose |
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