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发酵产氢细菌分离培养的厌氧实验操作技术
引用本文:李永峰,任南琪,陈瑛,李建政,胡立杰,郑国香.发酵产氢细菌分离培养的厌氧实验操作技术[J].哈尔滨工业大学学报,2004,36(12):1589-1592,1694.
作者姓名:李永峰  任南琪  陈瑛  李建政  胡立杰  郑国香
作者单位:哈尔滨工业大学,市政环境学院,黑龙江,哈尔滨,150090
基金项目:国家杰出青年科学基金资助项目(50125823),国家重点基础研究发展规划资助项目(G2000026402),哈尔滨工业大学跨学科交叉性研究基金资助项目(HIT.MD2002 25).
摘    要:厌氧产氢细菌的分离纯化与培养是发酵法生物制氢技术的基础课题之一,现有厌氧细菌的分离制备技术和培养基分别存在着操作繁琐和成份复杂问题.通过厌氧培养技术比较和细菌生长试验,确定了“煮沸吹氮去氧,固液交替分离”的厌氧产氢细菌分离纯化培养程序,改进了Hungate技术和建立了新的宽体窄颈培养瓶平板技术,通过培养基组成成份的增减和细菌增长、种类和数量的研究确立了厌氧发酵产氢细菌的分离和富集培养基.利用这些厌氧培养技术,已成功分离培养了550余厌氧菌株系.

关 键 词:生物制氢  产氢发酵细菌  分离纯化  厌氧操作  培养基
文章编号:0367-6234(2004)12-1589-04
修稿时间:2003年12月10

The experimental anaerobic operation of isolation and culture on hydrogen-producing and fermentative bacteria
LI Yong-feng,REN Nan-qi,CHEN Ying,LI Jian-zheng,HU Li-jie,ZHENG Guo-xiang.The experimental anaerobic operation of isolation and culture on hydrogen-producing and fermentative bacteria[J].Journal of Harbin Institute of Technology,2004,36(12):1589-1592,1694.
Authors:LI Yong-feng  REN Nan-qi  CHEN Ying  LI Jian-zheng  HU Li-jie  ZHENG Guo-xiang
Abstract:Isolation and culture of hydrogen-producing and fermentative bacteria is an important foundation on biohydrogen production process. There are complicated operation and composition in present anaerobic techniques and culture media. Hungate technique was improved and plate of culture bottle was established by comparing anaerobic methods and bacterium growth. Isolation and enrichment culture media were confirmed by the test of different composition and the species and amount of hydrogen-producing and fermentative bacterium.550 bacterium strains were isolated by the anaerobic operation.
Keywords:biohydrogen production  hydrogen-producing and fermentative bacteria  isolation and culture  anaerobic operation  culture media
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