| Related citation: | HOU Ning,YANG Ji-xian,MA Fang,LI Da-peng,WEN Qin-xue,XU Yang,GUO Jing-bo.Properties study and phylogenetic analysis of a bacterial strain with high de-emulsification efficiency[J].Journal of Harbin Institute Of Technology(New Series),2009,16(3):350-354.DOI:10.11916/j.issn.1005-9113.2009.03.010. |
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| Properties study and phylogenetic analysis of a bacterial strain with high de-emulsification efficiency |
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| Author Name | Affiliation | | HOU Ning | State Key Laboratory of Urban Water Resources and Environment,Harbin Institute of Technology,Harbin 150090
School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China | | YANG Ji-xian | State Key Laboratory of Urban Water Resources and Environment,Harbin Institute of Technology,Harbin 150090
School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China | | MA Fang | State Key Laboratory of Urban Water Resources and Environment,Harbin Institute of Technology,Harbin 150090
School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China | | LI Da-peng | State Key Laboratory of Urban Water Resources and Environment,Harbin Institute of Technology,Harbin 150090
School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China | | WEN Qin-xue | State Key Laboratory of Urban Water Resources and Environment,Harbin Institute of Technology,Harbin 150090
School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China | | XU Yang | State Key Laboratory of Urban Water Resources and Environment,Harbin Institute of Technology,Harbin 150090
School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China | | GUO Jing-bo | State Key Laboratory of Urban Water Resources and Environment,Harbin Institute of Technology,Harbin 150090
School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China |
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| Abstract: |
| For further practical application,the phylogenetic analysis and de-emulsification properties study of strain XH1 with high de-emulsification efficiency isolated from crude oil contaminated soil in Daqing oil field were conducted with a surfactant-stabilized water-kerosene model emulsion. The factors influencing the de-emulsification efficiency and the generation site of de-emulsification active component of the strain were also investigated. The similarity of 16SrDNA sequences between strain XH1 and Bacillus mojavensis(DQ993678)was 99%. According to the physiological biochemical test,strain XH1 was preliminarily identified as Bacillus mojavensis. The logarithmic growth,stable phase and decline phase of strain XH1 were determined as 14,18 and 28 h,respectively. The best de-emulsification activity emerged after cultivating for 18h,and the complete de-emulsification was achieved at 24 h. The most favorable incubation conditions for de-emulsification occurred with pH of 6.0 at 30 ℃. The de-emulsification capability of strain XH1 was mainly resulted from extracellular metabolites. The above results indicate that strain XH1 has high de-emulsification efficiency and is potential as a commercial de-emulsifier. |
| Key words: efficient bio-demulsification phylogenetic analysis demulsifying performance active site |
| DOI：10.11916/j.issn.1005-9113.2009.03.010 |
| Clc Number:X703 |
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