Please submit manuscripts in either of the following two submission systems

    ScholarOne Manuscripts

  • ScholarOne

    • 勤云稿件系统

  • 登录

Search by Issue

  • 2024 Vol.31
  • 2023 Vol.30
  • 2022 Vol.29
  • 2021 Vol.28
  • 2020 Vol.27
  • 2019 Vol.26
  • 2018 Vol.25
  • 2017 Vol.24
  • 2016 vol.23
  • 2015 vol.22
  • 2014 vol.21
  • 2013 vol.20
  • 2012 vol.19
  • 2011 vol.18
  • 2010 vol.17
  • 2009 vol.16
  • No.1
  • No.2

Search by Keywords

  • Search by Title
  • Search by Keywords
  • Search by Abstract
  • Search by Author's Chinese Name
  • Search by Author's English Name
  • Search by Fund

News & AnnouncementMORE

Supervised by Ministry of Industry and Information Technology of The People's Republic of China Sponsored by Harbin Institute of Technology Editor-in-chief Yu Zhou ISSNISSN 1005-9113 CNCN 23-1378/T

期刊网站二维码
微信公众号二维码
Related citation:SONG Jin-zhu,YANG Xiao-xue,Wang Yong,Yang Qian.Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana[J].Journal of Harbin Institute Of Technology(New Series),2009,16(4):453-456.DOI:10.11916/j.issn.1005-9113.2009.04.002.
【Print】   【HTML】   【PDF download】   【View/Add Comment】  【Download reader】  【 Close 】
←Previous|Next→ Back Issue    Advanced Search
This paper has been: browsed 710times   downloaded 0times 本文二维码信息
码上扫一扫！
Shared by: Wechat More
Font:larger+|default|smaller-
Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana
Author NameAffiliation
SONG Jin-zhu Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
YANG Xiao-xue Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Wang Yong Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Yang Qian Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Abstract:
To study recycled trashes from shrimps and crabs in the sea through chitinase secreted by microorganisms,the chitinase gene chit2 was cloned and sequenced from Beauveria bassiana by the polymerase chain reaction(PCR),and was ligated into the yeast expression vector pYES2.The expression vector plasmid was transformed into Saccharomyces cerevisiae H158.Gene expression took place upon induction with 2% galactose.The measurement of enzyme activity shows that the expression production can be expressed in active forms and secreted to the medium.The enzyme activity approaches the peak of 0.63 U/mL when the culture time is 36 h.
Key words:  Beauveria bassiana  chitinase  Saccharomyces cerevisiae  expression
DOI：10.11916/j.issn.1005-9113.2009.04.002
Clc Number:Q78
Fund:

LINKS

Copyright © The Editorial Department of Journal of Harbin Institute of Technology
Address: 92 West Dazhi Street, Nan Gang District, Harbin, Heilongjiang Province, China Postcode: 150001
(C)2015 JOURNAL OF HIT ALL RIGHTS RESERVED. ICP. 0000423255