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SONG Jin-zhu,YANG Xiao-xue,Wang Yong,Yang Qian.Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana[J].Journal of Harbin Institute Of Technology(New Series),2009,16(4):453-456.DOI:10.11916/j.issn.1005-9113.2009.04.002.

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Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana

Author Name	Affiliation
SONG Jin-zhu	Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China
YANG Xiao-xue	Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China
Wang Yong	Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China
Yang Qian	Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China

Abstract:

To study recycled trashes from shrimps and crabs in the sea through chitinase secreted by microorganisms,the chitinase gene chit2 was cloned and sequenced from Beauveria bassiana by the polymerase chain reaction(PCR),and was ligated into the yeast expression vector pYES2.The expression vector plasmid was transformed into Saccharomyces cerevisiae H158.Gene expression took place upon induction with 2% galactose.The measurement of enzyme activity shows that the expression production can be expressed in active forms and secreted to the medium.The enzyme activity approaches the peak of 0.63 U/mL when the culture time is 36 h.

Key words: Beauveria bassiana chitinase Saccharomyces cerevisiae expression

DOI：10.11916/j.issn.1005-9113.2009.04.002

Clc Number:Q78

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