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Supervised by Ministry of Industry and Information Technology of The People's Republic of China Sponsored by Harbin Institute of Technology Editor-in-chief Yu Zhou ISSNISSN 1005-9113 CNCN 23-1378/T

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Related citation:HeJie,GaoYong,SUN Ye-qing.Genomic and proteomic analysis of soybean heritable variations induced by space flight[J].Journal of Harbin Institute Of Technology(New Series),2009,16(4):587-592.DOI:10.11916/j.issn.1005-9113.2009.04.026.
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Genomic and proteomic analysis of soybean heritable variations induced by space flight
Author NameAffiliation
HeJie Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
GaoYong Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
SUN Ye-qing Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Abstract:
To analyze the biological effects of space environment,the diversity of genomic DNA between the space flight soybean 194(4126) with phenotype of good yield and good fruit quality induced by space flight and the soybean with ground control was studied by amplified fragment length polymorphism(AFLP) method,and the polymorphism of space flight soybean 194(4126) was 3.56%.The differences of protein expression of seeds and leaves between the two kinds of soybeans were analysed by two-dimensional electrophoresis,PDQuest software and MALDI-TOF mass spectrometry.Results show that the loss and decrease of protein expression in 194(4126) soybean are subjected to the space fight of seeds,and three special proteins including Dehydrin,MAT1 and ceQORH are identified.It is concluded that the space environment changes the phenotype and genotype of soybeans due to the space flight of seeds.
Key words:  space environment  genomic  proteomic  soybean
DOI：10.11916/j.issn.1005-9113.2009.04.026
Clc Number:S565.1
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