| Author Name | Affiliation | | CHANG Yu-guang | School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China
School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing 211171,China | | MA Fang | School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing 211171,China | | GUO Jing-bo | School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing 211171,China |
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| Abstract: |
| Strain HIT-3,which has good performances in cellulose degradation,was isolated from the campus soil. Based on the identification of conservative sequences 16S rDNA and the analysis of physiological-biochemical characteristics,HIT-3 was identified as Achromobacter xylosoxidans. Denitrificans. A two-step fermentation process was conducted by adopting compound-bioflocculant-producing flora constructed by cellulose-degrading bacterium HIT-3 and flocculating bacterium F2. The cellulose degradation metabolites of HIT-3 was taken as substrates by flocculating bacterium F2,by which excellent compound bio-flocculant was obtained. In addition,the enzymology characteristics of HIT-3 were investigated when cultured in cellulose media,which utilized CMC-Na as its sole carbon. The results show that HIT-3 achieves a climax of 67.6 U/mL of enzyme production after incubation for 6 d,and the organic carbons produced are sufficient as the substrates required by the fermentation of flocculating bacterium F2(flocculating efficiency of 85.6%),which makes it feasible to reuse bioenergy. |
| Key words: two-stage fermentation cellulose degrading bacterium flocculating bacterium cellulase activity |
| DOI：10.11916/j.issn.1005-9113.2010.03.005 |
| Clc Number:TQ925 |
| Fund: |
