| Related citation: | LIU Jian,LIU Chun-xiu,LUO Jin-ping,LIU Ru-ping,TIAN Qing,CAI Xin-xia.Imaging Escherichia coli using streptavidin functionalized quantum dots as a Nano-fluorescent probe[J].Journal of Harbin Institute Of Technology(New Series),2011,18(2):77-80.DOI:10.11916/j.issn.1005-9113.2011.02.016. |
|
| Author Name | Affiliation | | LIU Jian | School of Control Science and Engineering,Shandong University,Jinan 250061,China
State Key Lab of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100080,China | | LIU Chun-xiu | State Key Lab of Transducer Technology,Institute of Electronics,Chinese Academy of | | LUO Jin-ping | State Key Lab of Transducer Technology,Institute of Electronics,Chinese Academy of | | LIU Ru-ping | State Key Lab of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100080,China
Graduate University of Chinese Academy of Sciences,Beijing 100080,China | | TIAN Qing | State Key Lab of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100080,China
Graduate University of Chinese Academy of Sciences,Beijing 100080,China | | CAI Xin-xia | State Key Lab of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100080,China
Graduate University of Chinese Academy of Sciences,Beijing 100080,China |
|
| Abstract: |
| It is extremely important for bacteria detection in many fields,such as medical diagnosis and food safety.In this paper,streptavidin functionalized quantum dots(SA-QDs),as a nano-fluorescent probe,were used to attach with Escherichia coli(E.coli) for the detection and identification of bacteria with immunoreactions and biotin-streptavidin affinity.Fluorescent images of the bacteria and the fluorescence intensity were used to evaluate the conjugation effect with different incubation time.Our results showed that 20 min is a reasonable incubation time for the SA-QDs coupling to E.coli cells.The fluorescent images,which produce a greatly amplified and enhanced signal of E.coli cells,were obtained through the immunological amplification and fluorescent probe enrichment steps.In addition,the bleaching process of SA-QDs without any encapsulation at room temperature was clearly observed during 10 min of being excited.Our work provided a modularized sample treatment method using SA-QDs as a nano-fluorescent probe in cellular imaging and bio-labeling. |
| Key words: quantum dots immunoassay escherichia coli fluorescent probe imaging |
| DOI：10.11916/j.issn.1005-9113.2011.02.016 |
| Clc Number:Q93;O471.1 |
| Fund: |
