| Author Name | Affiliation | | Chang Sheng | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China | | LI Jian-zheng | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China | | LiuFeng | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China | | WANG Shu-jing | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China |
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| Abstract: |
| The effects of initial substrate (5-60 g /L) and biomass concentration (0.5-3 g /L) on fermentative hydrogen production by mixed cultures were investigated in batch tests using glucose as substrate.The experimental results showed that the hydrogen production increases as the initial substrate concentration increases from 0 to 25 g /L.It indicated that the shift in the metabolic pathway or in the composition of the bacterial flora occurs.The maximum hydrogen yield of 1.78 mol /mol-glucose is obtained at the substrate concentration of 15 g /L.This study also shows that initial biomass concentration affects the hydrogen yield as the cumulative hydrogen production has been increased with the increase of initial cell concentration up to 1.5 g /L and reached the highest level.The maximum hydrogen yield is obtained at the cell concentration of 1.5 g /L.It indicated that the optimum biomass /substrate ratio,maximizing the hydrogen yield and the hydrogen production rate,is determined to be 0.1 g biomass /g glucose. |
| Key words: fermentative hydrogen production hydrogen yield substrate concentration biomass concentration modified Gompertz model |
| DOI：10.11916/j.issn.1005-9113.2011.06.003 |
| Clc Number:X703 |
| Fund: |
