| Author Name | Affiliation | | Kai Wang | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China
School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, Shandong, China | | Peisheng Yan | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China
School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, Shandong, China | | Lixin Cao | School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, Shandong, China |
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| Abstract: |
| Chitinase is one of the most important mycolytic enzymes with industrial significance. Statistical methods are employed to optimize cultural conditions with the increased production of chitinase for the selected Serratia marcescens JPP1, which are obtained from peanut hulls in Jiangsu Province, China and exhibit antagonistic activity against aflatoxins. Using single-factor experiments the effects of cultural conditions (broth content, inoculum size and rotation speed) on chitinase production from S. marcescens JPP1 are evaluated. Central composite design of Response Surface Methodology is used to optimize the levels of factors for the best yield of enzymes production. The optimized cultural conditions for obtaining the highest level of chitinase production are 23.2 mL broth content, 116 r/min rotation speed and 4.3％ inoculum size. A quadratic regression model of chitinase production is built (R2=0.970 9) and the verification experiments confirm its validity. The maximum chitinase production obtained after the optimization is 29.58 U/mL for a 1.4-fold increase. |
| Key words: chitinase aflatoxin optimization Central Composite Design |
| DOI：10.11916/j.issn.1005-9113.2015.03.012 |
| Clc Number:X56 |
| Fund: |
