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Supervised by Ministry of Industry and Information Technology of The People's Republic of China Sponsored by Harbin Institute of Technology Editor-in-chief Yu Zhou ISSNISSN 1005-9113 CNCN 23-1378/T

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Related citation:Shuli Liu,Guangming Zhang.Isolation, Identification of Bacillus Thuringiensis/Cereus and Its Enhancement on Protein Wastewater Treatment by Rhodobacter Sphaeroides[J].Journal of Harbin Institute Of Technology(New Series),2016,23(5):69-75.DOI:10.11916/j.issn.1005-9113.2016.05.010.
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Isolation, Identification of Bacillus Thuringiensis/Cereus and Its Enhancement on Protein Wastewater Treatment by Rhodobacter Sphaeroides
Author NameAffiliation
Shuli Liu School of Environmental and Municipal Engineering, North China University of Water Resources and Electric Power, Zhengzhou 450000, China 
Guangming Zhang School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China
School of Environment and Resource, Renmin University of China, Beijing 100872, China 
Abstract:
In order to enhance the degrading protein capability of purple non-sulfur bacteria (PNSB), an effective strain, L2,was used to co-culture with Rhodobacter sphaeroides ATCC17023. The effects of added strain on protein removal of R. sphaeroides were investigated. Results showed that strain L2, being identified as Bacillus thuringiensis/cereus, had a high potential for producing protease with a production of 295 U/mL.The optimal B. thuringiensis/cereus(40 μL) could significantly increase protein degradation of R. sphaeroides. Protein removal and biomass production were improved by 483% and 67%, respectively. R. sphaeroides/total biomass production was more than 95%. Theoretical analysis revealed that R. sphaeroides syntrophically interacted with B. thuringiensis/cereus. Protein degradation of B. thuringiensis/cereus provided small molecule substrates (VFAs) for R. sphaeroides growth and cells materials synthesis.
Key words:  Rhodobacter sphaeroides  protein degradation  protease  Bacillus thuringiensis/cereus
DOI：10.11916/j.issn.1005-9113.2016.05.010
Clc Number:X703
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