| Related citation: | Kai Wang,Yawen Sun,Siqi Li,Yingjie Zhang,Xiquan Cheng.Biodegradation of Ammonia Nitrogen Using a Novel Candida sp. Strain N6 Immobilization[J].Journal of Harbin Institute Of Technology(New Series),2020,27(2):10-18.DOI:10.11916/j.issn.1005-9113.18125. |
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| Author Name | Affiliation | | Kai Wang | School of Marine Science and Technology, Harbin Institute of Technology, Weihai,Weihai 264209, Shandong, China
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China | | Yawen Sun | School of Marine Science and Technology, Harbin Institute of Technology, Weihai,Weihai 264209, Shandong, China | | Siqi Li | School of Marine Science and Technology, Harbin Institute of Technology, Weihai,Weihai 264209, Shandong, China | | Yingjie Zhang | School of Marine Science and Technology, Harbin Institute of Technology, Weihai,Weihai 264209, Shandong, China | | Xiquan Cheng | School of Marine Science and Technology, Harbin Institute of Technology, Weihai,Weihai 264209, Shandong, China |
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| Abstract: |
| Due to the high efficiency of ammonia nitrogen degradation, a novel Candida sp. strain N6 was applied for wastewater treatment by using immobilization technology. Immobilization conditions and corresponding ammonia removal capacity of immobilized N6 beads were investigated.The immobilized N6 beads were applied to degrade simulated ammonia nitrogen wastewater. Results showed that the optimum formula of immobilized materials was 9% polyvinyl alcohol, 1.5% sodium alginate, and 2% calcium chloride, under which the ammonia nitrogen removal rate of the immobilized N6 beads reached 97.97%. The following immobilization conditions were observed to be optimal: the immobilization time was 24 h, the inoculum of N6 was 3%, and the pH was 8.The immobilized N6 beads exhibited excellent ammonianitrogen degradation ability in SBRs. The ammonia nitrogen removal rate was stable at 95%-99% in the SBRs. The results indicated that immobilized N6 beads possess good application prospects in the treatment of ammonia nitrogen wastewater. |
| Key words: ammonia wastewater immobilization Candida sp. nitrification efficiency |
| DOI：10.11916/j.issn.1005-9113.18125 |
| Clc Number:X172 |
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| Descriptions in Chinese: |
| 固定化假丝酵母菌株N6降解氨氮的研究 王 凯1,2，孙亚文1，李斯琪1，张瑛洁1，程喜全1，马 军2 （1. 哈尔滨工业大学(威海)， 海洋科学与技术学院，山东 威海 264209； 2. 哈尔滨工业大学 市政与环境工程学院，哈尔滨 150090） 创新点说明：发现一株假丝酵母菌株，具有较高的氨氮降解能力，采用固定化技术，将其固定化后用于模拟生活污水的处理效果仍然显著，表明将该菌固定化后具有较高的应用价值。 研究目的： 将高氨氮处理效率的菌株N6固定化，以期通过固定化的形式将其应用于废水处理中。 研究方法： 从山东威海金海湾排污口分离出一株假丝酵母菌，该菌株具有较高的氨氮处理效率。采用包埋固定化技术将N6固定，考察了固定化N6的氨氮降解能力以及稳定性，通过单因素以及正交试验确定了固定化材料以及最佳固定化条件。利用SBR反应器探究固定化N6的应用情况。 研究结果： 1）当固定化材料PVA, SA, CaCl2的浓度分别为9%, 1.5%, 2%时最适合N6固定化，在此条件下，固定化N6的氨氮去除率达到了97.97%。 2）稳定性测试表明固定化N6具有很强的稳定性，固定化小球在测试条件下未出现破碎现象。 3）固定化N6的最佳固定化条件为：固定化24h，N6接种量3%，pH 8。 4） 固定化N6在SBR反应器中表现出非常好的氨氮降解能力，去除率稳定在95%-99%。 5）在固定化N6的应用过程中，最适添加量为15%。 结论： 固定化高效菌株用于废水处理具有广阔的前景。高氨氮处理效率的假丝酵母N6固定化后氨氮降解能力仍然较强。固定化N6具有潜在的应用价值，将固定化N6应用到SBR中，具有较高且稳定的氨氮去除率。 关键词：氨氮废水；固定化；假丝酵母菌属；脱氮效率 |
