To control agricultural non-point source pollution, the cleaning biological fertilizer and chemical fertilizer were used together to raise nitrogen use efficiency. The growth and production of rice under the conditions of single application of nitrogen fertilizer(N), single application of biological fertilizer with Glomus mosseae as the subject(M) and joint application of nitrogen fertilizer and biological fertilizer(NM) were studied, and the results reveal: (1)The application of nitrogen and G.mosseae(NM) makes rice reach the highest net photosynthetic rate, and increases by 4.5％ and 5.0％ more than N and M treatment, respectively. (2) The nitrogen fertilizer can increase the aboveground biomass by 22.8％, the biological fertilizer can increase the underground biomass by 41.5％ significantly (P＜0.05), and NM treatment can promote both the above-and under-ground biomass (37.0％ and 35.0％, respectively). (3) The NM treatment increases rice yield by 4.8％ more than traditional nitrogen fertilizer management. Therefore, mycorrhizal infection to the rice can effectively improve the efficiency of resource utilization, growing and producting capacity.