The mistuned stiffness model of bolted rotor with curvic couplings was established, and the effect of bolt preload mistuning on dynamic characteristics of the rotor was studied. At last, the dynamic response of the rotor was applied to finite element model as displacement boundary conditions to analyze the dynamic stress distribution under the condition of dynamic bending, and the influence of bolt mistuning on stress distribution of curvic couplings was obtained. The results show that the stiffness anisotropy of the rotor can be reduced when positive mistuning bolt lies on the major axis of the running orbit, or negative mistuning bolt lies on the minor axis. The stress variation of each tooth can be reduced if negative mistuning bolt is on the compression side, and it will be further increased if negative mistuning bolt is on the tensile side when dynamic bending happens, whereas the stress change tendency of curvic teeth is opposite under the positive mistuning condition.