To investigate the effect of different environmental factors on the efficiency of denitrifying phosphorus removal bacteria (DPB), anaerobic/oxic/anoxic reactor (A/O/A-SBR) was used to culture the granular sludge. By designing the orthogonal test, the overall investigation of the biological nitrogen and phosphorus removal efficiency of DPB under various carbon source, carbon concentration, inlet water temperature and pH value was carried out. The results show that: when carbon source is sodium propionate, COD concentration is 400 mg/L, inlet water temperature is 25 ℃, and pH value is 7, the COD degradation efficiency of DPB is the highest; when carbon source is sodium propionate, COD concentration is 400 mg/L, inlet water temperature is 15 ℃, pH value is 7, the nitrogen removal efficiency of DPB is the highest; when carbon source is sodium acetate, COD concentration is 400 mg/L, inlet water temperature is 25 ℃, pH value is 8, the removal efficiency of soluble P is best. Temperature is the most influential factor for the COD and nitrogen removal, while the influence of pH is the minimum. The most influential factor for phosphorus removal of DPB is pH, and carbon source affects the minimum.