To solve the problem of agglomeration, low catalytic activity and difficulty to recycle and utilize of the 1-D, 2-D nanomaterials during the process of degradating organic pollutants.3-D TiO₂ nanoflowers with chrysanthemum structure were prepared by template hydrothermal method in which TiO₂(P25)and NaOH as raw materials, with assistance of ZnO nanostructure templates. Petals in nanoflowers were self-assembled and arranged directionally by TiO₂ nanoparticles.The TiO₂ nanoflowers was characterized by scanning electron microscopy(SEM), X-ray diffraction(XRD), nitrogen adsorption-desorption, ultraviolet-visibles diffuse reflectance sepctroscopy (UV-vis DRS), Fourier transform infrared (FTIR). The SEM images indicated that TiO₂ nanoflowers were chrysanthemum shape and average particle size was 5 μm. Self-assembled nanoflower of the diameter of the nanoparticles was about 15 nm. It was confirmed by XRD that the TiO₂ nanoflowers was pure anatase crystalline structure with good crystallinity. The N₂ adsorption-stripping isotherm results showed that specific surface area was 102.3 m²／g and average pore size is about 17.41 nm. Methylene blue as the target pollutant, the photocatalytic degradation of methylene blue rate in 80 minutes could reach 98％ under UV irradiation. So photocatalytic activity of TiO₂ nanoflowers was higher than P25, and showing stronger photocatalytic degradation ability.