To investigate the inhibitory effect of polysaccharides from corn bran extracted by three different methods on the HT-29 cells, it was studied by MTT assay, inverted microscope, mitotic index and colony formation. MTT results showed that the water extraction polysaccharide from corn bran had a significant inhibition effect on the proliferation of HT-29 cells in a dose-dependent manner. After treatment for 48 h with 0.02 g／L of water extraction polysaccharide from corn bran, the inhibitory rate was 60.62％. The other two polysaccharides had no affect on the growth of HT-20 cells. The morphologic observation showed that the water extraction polysaccharide from corn bran induced obvious morphological changes. The mitotic index and colony formation was decreased compared with the control group as the incubation time and concentration of water extraction polysaccharide from corn bran.