With the improvement of urban planning, surface processing industrial parks are continuous built in China.Due to its complex water quality and abundant existence of heavy metals, the traditional biological treatment unit exposed serious threaten from the existed heavy metals.Thus, the most common cation in electroplating wastewater, Cu²⁺, was chosen as object and the tolerant bacteria were isolated for enhancing its bulk removal.Two bacteria strains that have strong tolerance ability to Cu²⁺ were isolated and named as L2 and L3, and the effect of adsorption time, pH, temperature and initial concentration on Cu²⁺ removal were investigated. Results showed L2 and L3 have a high Cu²⁺ activity both in LB medium and wastewater medium, and the adsorption of Cu²⁺ could be achieved in a short time, with an optimal pH of 6.0 and temperature of 28℃, respectively.Under the optimal condition, the maximum adsorption ability was 34.15 mg/g Cu²⁺ for L2 and 45.68 mg/g for L3. A fast and efficient removal of Cu²⁺ can be realized, which is anticipated to be used in the actual electroplating wastewater treatment.