Atrazine residue in water poses a serious threat to the environment and human health. Phytoremediation stood out as a potential technology for in-situ remediation of atrazine in the environment. A pot culture experiment was conducted to investigate the effects of arbuscular mycorrhizal fungi (AMF) on the phytoremediation of atrazine by Canna indica L. var. flava Roxb. The results demonstrated that arbuscular mycorrhizal fungi formed a stable symbiosis with canna a month after its inoculation, and the root colonization rate was (22.1±4.6)%. Arbuscular mycorrhizal fungi relieved the inhibition effect of atrazine on canna growth, and improved the plant height, root length and biomass of canna under the stress of atrazine; mycorrhizal dependency on the growth of canna was significant, and achieved the maximum value at 5 mg·L^-1 concentration of atrazine. Inoculation of arbuscular mycorrhizal fungi significantly increased the canna phytoremediation degradation rate of atrazine, and reduced the half-life of atrazine; furthermore, inoculation of arbuscular mycorrhizal fungi improved the atrazine concentration range of canna phytoremediation. This study shows that canna inoculated with arbuscular mycorrhizal fungi is an ideal choice of atrazine pollution remediation, and may ultimately serve as a viable phytoremediation solution for in situ remediation of atrazine in water.