Lab-scale simultaneously anaerobic ammonia oxidation and denitrification (SAD) process was operated in Municipal Waste Water Treatment Plant (WWTP). Sewage treated by A/O and nitrification process was served as substance to start up anaerobic ammonia oxidation filter reactor. Adding organic carbon-source to influent was served as substance to start up SAD filter reactor after successful start-up of Anammox reactor. Glucose was selected as organic carbon-source because it is harmless and inexpensive. The result show that SAD process performed well with 10, 20, and 30 mg/L Glucose added to effluent sewage at ambient temperature and average total nitrogen concentration of effluent was 9.16, 8.10, and 6.41 mg/L. Compared with Anammox process, SAD process performed better and total nitrogen concentration in effluent decreased 16-42 percents. Stability of SAD process was destroyed and SAD process turned to denitrification process when 30 mg/L Glucose was added in influent sewage in winter. Ammonia in effluent increased from 0.5 mg/L to 6.2 mg/L. The results indicate that temperature has superior effect on SAD process. The total nitrogen concentration in effluent varied from 6.5 to 8.5 mg/L when concentration of Glucose decreased to 20 mg/L. In winter, the effluent of SAD process reached the 1A level of integrated discharge standard of water pollutants applied in Beijing City.