In order to obtain the wind velocity histories of Hutong rail-cum-road Yangtze River Bridge, an in-situ wind field test of the cable-stayed bridge was carried out. The wind field characteristics were analyzed, and the wind velocity spectra parameters of the experiment were compared with those of the specification. By means of harmonic synthesis method, this paper simplified the 3D turbulence wind field into multi 1D turbulence wind filed, and simulated the turbulence wind velocity histories of two towers, the girders on roadway and on railway deck separately. Results showed that the 3D turbulence wind velocities obtained based on in-situ test records fitted well with the target value, and exhibited differences with the specification values. It indicates that the application of specification spectra values is limited when used in different regions, especially in regions with complex climate conditions. The wind velocity histories of the long-span cable-stayed bridge obtained can be used in bridge aerodynamic analysis, which is pragmatic for engineering purposes.