To solve the problems that fabric/paper has many surface defects and poor bonding with conductive ink, a more universal method, i.e., PVA modification and PVC modification, as well as conductive silver ink was adopted to prepare fabric/paper-based flexible electronic films with a sheet resistance of less than 10 Ω. The scanning electron microscope was applied to characterize the original morphology of the substrate, the modified layer in the middle, and the sintered layer of silver particles on the surface. The four-probe tester was used to characterize changes of sheet resistance with increasing bending times, tearing times, and immersion time. The flexible electronic film still showed excellent electrical conductivity after being bent for 100 000 times, immersed for 120 hours, and torn for more than 20 times. Effects of screen printing process parameters on printing accuracy were explored, and common printing defects of screen printing were summarized. The application value of flexible electronic thin films was preliminarily discussed. LED array circuit was designed and printed. Temperature and strain distributions of the printed circuit board surface mount resistor during operation were modeled and simulated. Screen-printed electrode for glucose detection was designed and prepared. Printed electrode has excellent electron transfer performance and better detection performance for glucose detection.