In order to investigate the temperature evolution of reinforced concrete top-head platform of metro under fire conditions and its impact on the structure, a scale model test was carried out under vehicle fire. The temperature evolution and distribution of the envelope and indoor space of the model room were measured. On this basis, finite element simulation analysis of the top-head floor was performed combined with layered shell model. Results show that the structure maintained intact after fire, which meets the fire resistance requirements. In addition, a high-temperature thermo-mechanical coupling calculation model for reinforced concrete floor was established, and its simulation results agreed well with test results, which further verified the reliability of the proposed method. The study can provide reference for the fire resistance evaluation of top-head platform of metro.