In order to ensure the anti-glare effect of plants and avoid the formation of “wall effect”, from the perspective of road alignment combination, considering factors such as the number of lanes, vehicle type, and drivers vision, firstly, spacing models for plants on straight and curved sections, height models for superelevation or not superelevation horizontal curve, height models for concave and convex vertical curves, and a plant height limit model were constructed. Secondly, the models were calibrated based on the specifications and commonly used parameters in actual construction, and the theoretical values of the spacing and height at different speeds were obtained. Considering the cost of plants and the convenience of construction and maintenance, the recommended values were determined. Finally, taking Heida Highway as an example, the spacing and height models of plant anti-glare were validated, and the results showed that at a speed of 100 km/h, the spacing is 9 m and the height is 1.90 m in straight slope and straight concave vertical curve sections; The spacing and height of the superelevation horizontal curve section are 3 m and 1.95 m respectively; The spacing in convex vertical curve sections is 3 m, and the height is 1.90 m. The recommended values meet the design requirements for differentiation and precision under different alignment combination, while also facilitate construction and maintenance, and reduce costs.