To alleviate driving fatigue caused by driving on grassland roads, an anti-fatigue warning strategy based on information demand of individual drivers was designed. Based on the questionnaire, drivers were divided into three categories: visual type, auditory type and tactile type. Considering different types of drivers′ customized warning information demand, three kinds of driving fatigue warning strategies on grassland roads were designed, including road warning, vehicle warning and vehicle-road cooperation warning. 21 drivers were selected to carry out driving simulator experiment. The data of KSS scale, subjective evaluation of warning strategy and driving behavior were obtained. The methods of descriptive analysis, statistical analysis and TOPSIS comprehensive evaluation based on entropy weight were applied to test the effectiveness of three driving fatigue warning strategies. The results shown that drivers′ acceptance of the three warning strategies was high; these three warning strategies could alleviate the driving fatigue status in different degrees, the cooperative warning strategy was superior to the other two strategies. Moreover, the alleviation effect of the cooperative warning strategy was more obvious when the driving fatigue degree was deeper. The cooperative driving fatigue warning strategy based on customized information demand of individual drivers was helpful to improve the prevention effect of driving fatigue on grassland roads.