| 引用本文: | 李冬,张复旦,张杰,李帅,柴晨旭.厌/缺氧段HRT对平衡EDPR系统内DPAOs和DGAOs的影响[J].哈尔滨工业大学学报,2025,57(5):1.DOI:10.11918/202401035 |
| LI Dong,ZHANG Fudan,ZHANG Jie,LI Shuai,CHAI Chenxu.Effect of HRT in anaerobic/anoxic segment on balancing DPAOs and DGAOs within EDPR system[J].Journal of Harbin Institute of Technology,2025,57(5):1.DOI:10.11918/202401035 |
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| 厌/缺氧段HRT对平衡EDPR系统内DPAOs和DGAOs的影响 |
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李冬1,张复旦1,张杰1,2,李帅1,柴晨旭1
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(1.水质科学与水环境恢复工程北京市重点实验室(北京工业大学),北京 100124; 2.城市水资源与水环境国家重点实验室(哈尔滨工业大学),哈尔滨 150090)
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| 摘要: |
| 为解决反硝化除磷技术中反硝化聚糖菌（DGAOs）和反硝化聚磷菌（DPAOs）竞争碳源造成除磷性能恶化的问题,试验设置3组同规格的SBR反应器,通过对比不同厌/缺氧段HRT下的运行情况,探究各系统内碳源转化、脱氮除磷性能及DPAOs和DGAOs丰度比的变化。结果表明:厌/缺氧段HRT为90 min/170 min时,DGAOs与DPAOs丰度之比为1.97,内碳源储量（182.81 mg/L）和释磷量（31.72 mg/L）最大,COD、TP和NO-2-N去除率分别为94.69%、96.37%和90.40%；厌/缺氧段HRT为50 min/210 min时,厌氧时间过短导致微生物吸收碳源不充分,内碳源储量（141.59 mg/L）最低,同时,缺氧时间过长导致DGAOs因储存的糖原（Gly）被反硝化利用而影响生长,DGAOs与DPAOs丰度之比最低（0.49）；厌/缺氧段HRT为130 min/130 min时,DGAOs与DPAOs丰度之比升至2.63,厌氧时间过长不利于DPAOs储存内碳源,出水TP大于0.5 mg/L,同时,缺氧时间过短不利于反硝化作用,NO-2-N去除率降至81.05%；在50 min/210 min时,DPAOs占比较高更有利于PN分泌,促进成粒（平均粒径为517.6 μm）,130 min/130 min时DGAOs占比更大促使PS分泌,不利于成粒（平均粒径为255.3 μm）；厌/缺氧段HRT为90 min/170 min时,污泥平均粒径为480.1 μm,此时建立的DGAOs-DPAOs平衡,系统稳定性和污染物去除性能均为最佳。 |
| 关键词: 厌/缺氧段水力停留时间 反硝化聚磷菌 反硝化聚糖菌 内源反硝化除磷 高通量测序 |
| DOI：10.11918/202401035 |
| 分类号:X703.1 |
| 文献标识码:A |
| 基金项目:北京高校卓越青年科学家计划(BJJWZYJH 01201910005019) |
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| Effect of HRT in anaerobic/anoxic segment on balancing DPAOs and DGAOs within EDPR system |
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LI Dong1,ZHANG Fudan1,ZHANG Jie1,2,LI Shuai1,CHAI Chenxu1
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(1.Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering(Beijing University of Technology), Beijing 100124, China; 2.State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology), Harbin 150090, China)
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| Abstract: |
| In order to address the issue of deterioration of phosphorus removal performance caused by competition between denitrifying glycogen-accumulating organisms (DGAOs) and denitrifying phosphorus-accumulating organisms (DPAOs) for carbon source in denitrification and phosphorus removal technology, three groups of the same specification of SBR reactor were set up in the experiment. Changes in the conversion of internal carbon source, denitrification and phosphorus removal performance as well as the ratio of abundance of DGAOs to that of DGAOs were explored by comparing the operation under the different anaerobic/anaerobic sections with different HRTs. The results show that with an anaerobic/anoxic HRT of 90 min/170 min, the abundance ratio of DGAOs to DPAOs is 1.97, with the maximum internal carbon storage (182.81 mg/L) and phosphorus release (31.72 mg/L). The removal rates of COD, TP, and NO-2-N are 94.69%, 96.37%, and 90.40%, respectively. Conversely, with an anaerobic/anoxic HRT of 50 min/210 min, the insufficient anaerobic time results in inadequate uptake of carbon by the microorganisms, with the lowest endogenous carbon storage (141.59 mg/L). Additionally, the prolonged anoxic time causes DGAOs to utilize stored glycogen (Gly) for denitrification, adversely affecting their growth and resulting in the lowest abundance ratio of DGAOs to DPAOs (0.49). When an anaerobic/anoxic HRT of 130 min/130 min, the abundance ratio of DGAOs to DPAOs increases to 2.63. However, the excessive anaerobic time detrimental to the storage of the internal carbon source of DPAOs, resulting in effluent TP levels exceeding 0.5 mg/L. Additionally, the insufficient anoxic time negatively impacts denitrification, causing the removal rate of NO-2-N decrease to 81.05%. At an HRT of 50 min/210 min, a higher proportion of DPAOs is more conducive to PN secretion, promoting granulation with an average particle size of 517.6 μm. In contrast, the larger proportion of DGAOs at 130 min/130 min enhances PS secretion, which is not conducive to granulation, resulting in a smaller average particle size of 255.3 μm. At an anaerobic/anoxic HRT of 90 min/170 min, the average particle size of the sludge is 480.1 μm, establishing a balance between DGAOs and DPAOs, leading to optimal system stability and pollutant removal performance. |
| Key words: anaerobic/anoxic segment HRT denitrifying phosphorus-accumulating organisms (DPAOs) denitrifying glycogen-accumulating organisms (DGAOs) endogenous denitrification for phosphorus removal high-throughput sequencing |
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