The combination of chromatogram and chemical analysis was adopted to optimize the methods selected to measure poly-P,glycogen and PHA intracellular polymer in EBPR system.PHA was measured by GC system,glycogen was degraded by dilute hydrochloric acid and analyzed by glucose commercial kit,and poly-P was oxidized by potassium persulfate and analyzed by molybdenum-antimony anti-spectrophotometric method.Testing of the proposed method for PHA analysis shows that 4% sulfuric acid concentration and degradation for 20 h are required for optimal PHB and PHV extraction,while 0.6 mol/L HCl and digestion for 5 h produce the highest recovery of glycogen,and the medical glucose kit can be used in this research.Oxidation for 30 min at 120 ℃ is recommended for the recovery of poly-P.The results of precision analysis and recovery test with standard addition of these methods show that they possess high precision and are convenient for laboratory research and practical application.