In order to investigate the inhibitory effect of antibiotic on anaerobic digestion of piggery wastewater methane production was measured in batch experiment with aureomycin as inhibitor.Results showed that the maximum methane production rate of simulative wastewater was 19.6 mL/(L·h).In the presence of 10 mg/ L of aureomycin,the maximum methane production rate was reduced by 47.4% .The hydrolysis and hydrogen-producing were more inhibited than methane producing process in this experiment,which was supposed to be the sensitive step in anaerobic degradation of piggery wastewater treatment.As aureomycin in piggery wastewater is inhibitory to anaerobic,digestion measures should be taken to strengthen the hydrolysis and hydrogen producing process.