In order to promote the engineering applications of anaerobic ammonium oxidation (ANAMMOX) for urban sewage treatment,the impact of nitrate,phosphate,ammonia and nitrite on the running performance of ANAMMOX biofilter reactor at room temperature was investigated respectively. Experimental results show that there is no significant impact on the TN removal load when the influent NO 3--N concentration is increased to about 500 mg/L. However,the TN removal load will drop obviously when the infulent TP concentration is higher than 10 mg/L,which can be restored after stopping dosing phosphate. Appropriately increasing the concentration of NH 4+-N and NO 2--N is beneficial to the improvement of TN removal load. At room temperature conditions,nitrate basically has no effect on the ANAMMOX biofilter for municipal wastewater with low ammonia. But for phosphate,it has a certain impact on the activity of ANAMMOX,and the improvement of the influent phosphate concentration has a reversible inhibition for ANAMMOX reaction in the urban sewage with low ammonia.