Aniline-degrading bacteria conserved by laboratory were tested in Sequencing Batch Reactor(SBR) to investigate the capability of aniline removal,the variation of interrelated index such as COD,NH 4+ ,NO 2-and NO 3-,and the community succession of microorganism. The results showed that SBR started up quickly and degraded aniline effectively. The removal efficiency of aniline reached 100% and the removal efficiency of COD was about 93% when the system reached steady running. The photographs screened by scanning electron microscope(SEM) indicated that the aniline-degrading bacteria spread over the activated sludge. It was found from the PCR-DGGE map and the similarity matrix that the species and the amounts of microorganism changed greatly,and dominant species in the system were strengthened.