The effects of inoculating arbuscular mycorrhizal fungi (AMF) on the growth of rice and the reduction of chemical fertilizer usage were investigated in pot experiments,which included substrates with three different AMF concentrations. The inoculation situations of AMF to the roots of rice were compared,and the promotion to the rice growth and the potential of AMF in reducing the agricultural non-point pollution generated by chemical fertilizer were also investigated. The results showed that G. mosseae was ubiquitous in the natural substrate and could formulate mycorrhizal with the root system of rice. However,the effects of blank controller and natural substrate on the growth of rice did not have significant statistical difference. AMF could obviously enhance the mycorrhizal colonization rate to the root system of rice. Compared with the natural substrate,the stem lengths,total biomass,surface areas of root system and the yield of rice were enhanced by 20. 6% , 30% ,36. 6% and 45. 3% ,respectively. 3. 14 kg nitrogen fertilizer,1. 88 kg phosphate fertilizer and 2. 42 kg potassic fertilizer were reduced for producing per 100 kg rice. AMF enhanced the growth and production of rice and was potential in reducing the pollution caused by the usage of chemical fertilizer.