For determining the pollution status of endocrine disrupting compounds(EDCs) in Songhua River, solid phase extraction (SPE) followed by GC-MS／Yeast Estrogen Screen(YES) assay were used to analyze the EDCs concentration at 11 sampling sites of Songhua River. The results indicated that total five estrogen ∑5ES[JB))] was in the range of 6.59-30.97 ng／L, the average value was 17.23 ng／L, and the water from Dalian river outfall had the highest ∑5ES. Estrogen (E₁) and 17β-estradiol (E₂), which were mainly from urban sewage and contributed most of all estrogen and their concentrations, were in the range of 1.54-6.34 and 1.86-20.78 ng／L, respectively; Nonylphenol (NP), octylphenol (OP) and bisphenol A (BPA) were detected in all the sampling sites, with the mean values of 512.8,4.1 and 52.8 ng／L, respectively, which were mainly from the domestic sewage and industrial wastewater; The estrogenicity of river water detected by YES was in the range of 0.50-26.16 ng／L, and the highest EEQ occurred at the Haxia sampling site. The EEQ at other sampling site was all below 3.60 ng／L, which was at the same pollution level with other river, but it also existed the potential problems of ecological security in Songhua River.