To explore the effect of different AMF microbial inoculum on the growth of Trifolium repens lawn, we chose two kinds of widely used strains, Glomus mosseae and Glomus intraradices, to study the promoting effect of single bacterium, mixed bacteria on Trifolium repens respectively. The results show that:Simple infliction of Glomus mosseae could obviously improve the infection rate to 53.84％;Trifolium repens has evident dependence on fungicides especially on mixed fungicides. Besides, the dependence of plant aerial parts are obviously higher than underground ones; AMF has apparent promotion on the growth rate of Trifolium repens. The mixed fungicides are better than single ones, the growth rate of plant height, the overground and underground biomass liveweight could be improved by 40.7％, 76.6％, 35.7％ respectively; AMF could effectively enhance the Trifolium repens’ absorption capabilities of nutriment in rhizosphere soil, improve the utilization rate of hydrolysable nitrogen, available phosphorus to 51.6％, 48％. Hydrolysable nitrogen is the main inhibiting factor restraining fungicide's promotion on the growth of Trifolium repens with microbial inoculum, the phosphatic fertilizer is not needed, but a few nitrogen fertilizer is.