In order to improve the RFID system read efficiency, a reading mode named UID-Listed Reading Mode is proposed. With putting the UID of tags to be identified into the address list of a RFID reader, which was formed in advance, the address list would be tracked and searched until all of the tags were identified one by one, according to certain rules. To prove the strong points of the UID-Listed reading mode proposed, query times, transmission delay and system efficiency were simulated, and then contrasted with the Binary Search Algorithm, which had similar operation principle with proposed reading mode. The consequences of simulation indicate that the UID-Listed reading mode turns out to be better than the classical binary search algorithm.