This paper described the developments in the methods of EDCs detection. Biologically based assays, which comprise whole organism assays, cellular bioassays and non-cellular assays, seem to be promising methods for the determination of endocrine activities. On the other hand, the mass-based analytical methods show excellent sensitivity and precision for EDCs’ quantification. Several extraction techniques for the instrumental analysis have been developed since they are crucial in determining the overall analytical performances. To achieve both quantitatively and qualitatively successful analysis for EDCs, a combined approach incorporating two types of analyses was proposed, known as a bioassay-directed chemical analysis (BDCA) method. Further studies on EDCs need to be conducted, such as source reduction, limiting exposure to vulnerable populations, remediation of contaminated sites, and the detailed understanding of transport mechanisms in the environment.