A precise assembly and measurement system was developed and fabricated for multi-peg-holes assemble based on interference fit, in which a compliant gripping mechanism was designed to avoid the assembly jam, the force/stiffness position compensation was proposed to eliminate the fabrication tolerance deflection of 10 μm, and the ring components were precisely positioned in the assembly mold. Borescope decouple vision measurement was proposed to measure the position of assembled components in narrow and dark environment. The position information of assembled components was measured combining the information of captured image and grating rule which assembled on the linear guide driving the borescope. The experimental results show that using the developed system, the position errors of assembly were from -9.4 to 6.1 μm and the measurement errors were from -1.7 to 8.2 μm, it could meet the requirement of the precise assembly and measurement.