To improve the growth and survival rate of plants in vegetation-growing concrete, we chose two kinds of arbuscular mycorrhizal fungi, Glomus mosseae and Glomus intraradices, to study the effect of pure fungi, mixed bacteria on Poa pratensisl. cv. BALIN in vegetation-growing concrete. The results show that: The single application of Glomus mosseae (GM) can obviously improve the infection rate to 43.27％. Poa pratensis has stronger dependence on Glomus mosseae and mixed fungicides (MI) with little difference than Glomus intraradices (GI). The plant height and aboveground biomass with MI treatment can be increased by 15.8％ and 20％ respectively. Mtreatment can increase the underground biomass by 48％ higher than any other treatment. M treatment can significantly improve the proline and soluble sugar contents by 45.4％ and 27.7％ and reduce the malondialdehyde content (MDA) by 14.7％ which can enhance the plant stress-resistance. M treatment can make the plants get the highest CO₂ assimilation and water use efficiency (WUE), enhancing Poa pratensis’ ability of using the light and water and improving better growth of plants. Glomus mosseae is the best choice to improve vegetation-growing concrete.