To accurately and real-time reproduce complex electromagnetic space environment of aeronautical communications system data link, the dynamic interference was simulated. A semi-physical simulation system is designed to simulate the real flight’s anti-interference in static microwave chamber, then the characteristics of aeronautical communications system’s data chain is analyzed, when reproducing the dynamic interference in the anechoic chamber, the electromagnetic space environment’s dynamic interference mapping is achieved through the control of microwave instruments. The corresponding dynamic interference model is built in real-time by using the parametric model characterization techniques, and its matching output switching interference algorithm is designed by using the least mean square method. Thus, the function of simulating the dynamic interference through a static radio trumpet in the microwave chamber is realized by the method of combining static and dynamic simulation. Finally, the verification for anti-interference performance shows that the dynamic interference’s impact on the aircraft can be realistically reflected by the method of simulating dynamic interference in the anechoic chamber based on the microwave switch. The study of the simulation system can greatly improve the anti-interference performance of aeronautical communications system data chain.