In order to investigate the anoxic biodegradability and degradation pathway of three typical organic pollutants (quinoline, pyridine and catechol) in coal gasification wastewater, the acclimated anoxic activated sludge used as seeded sludge with NO₃--N as electron acceptor, and the anoxic degradation intermediates of the three pollutants were determined by UV-Vis and GC/MS analysis. Results showed that the inhibitory and toxic effects of the three compounds strengthened with the increase of the initial concentration. The residual concentration with the of its initial concentration 48 h anoxic treatment. The anoxic degradation rate constants of the three pollutants in descending order were pyridine, catechol and quinoline. The pollutants were partly converted into intermediates which were more refractory than the original compounds, instead of being mineralized to CO₂ and H₂O. The anoxic biodegradability of these pollutants enhanced with the addition of glucose as co-substrate. The co-metabolism effects were more significant when the pollutants were more refractory. The degradation of quinoline and pyridine initiated with the hydroxylation reaction. The possible degradation pathways were proposed based on the intermediates analysis. QSBR studies of the above three compounds showed that biodegradation rate constant (K_s) was significantly correlated with molecular connectivity indices ¹X^v as well as highest occupied molecular orbital EHOMO.