The feasibility of CANON granular sludge for efficient nitrogen removal from domestic wastewater was investigated in SBR. Firstly, CANON granular sludge was started by stirring under high ammonia concentration conditions, then granular sludge was increasingly added to improve the performance of nitrogen removal. Finally, the feasibility for domestic wastewater treating was investigated. The results show that CANON granular sludge could start-up within 40 days, the size of granular sludge reached 760 μm and the total nitrogen removal rate reached 1.01 kg/(m³d) within 126 days. A linear relationship between the ratio of polysaccharide and protein and the particle size growth was found. The total nitrogen removal rate increased rapidly to 3.22 kg/(m³d) within 77 days by adding granular sludge. Simultaneous removal of nitrogen and COD proved that the CANON granular sludge could be applied as an efficient approach for treating domestic wastewater.