To meet the requirements of driving and mixing on the microfluidic devices for chemical analysis, cell culture, and pharmaceutical test, multi types of AC electrothermal pump with mixing function were developed. A three-dimensional model was built for the study on the qualitative analysis for transport and mixing efficiency of the multi types of AC electrothermal pump with different driving voltages. We found that the electrodes with deflection angles have the best mixing efficiency. The results indicate that the new types of the asymmetric electrodes could have both functions of transport and mixing. At the limited size of microchannel, the electrodes with deflection angles of 25° were more outstanding on mixing than any other types, and the type of the electrodes with deflection angles is the best type in mixing efficiency. The velocity of 69.53 μm/s could be driven at the applied voltage of 7.070 V for this type, and it was 3.5％ faster than the velocity of the traditional micropump with asymmetric electrodes (67.16 μm/s).