To further unravel dynamic adsorption behavior of bovine serum albumin on the PVDF ultrafiltration membranes modified with three different additives (PVP, PVA or PEG), the membranes were fixed upon clean crystal by spin-coating, and the adsorption and cleaning of protein on different membrane surface were measured using quartz crystal microbalance with dissipation monitoring. It was found that the adsorption amount of BSA on PVDF/PEG membrane was relatively low, and the adsorbed layer structure was dense and difficult to remove by DI water cleaning. While for PVDF/PVP and PVDF/PVA membrane surfaces (especially PVDF/PVA), the conformation of the layers varied on later stage obviously, |-ΔD/Δf| values increased seriously, and the adsorption layers become looser. The results show that the absorbed mass of BSA is related to the hydrophilicity of membrane surfaces using the same additives, and the membranes modified with different additives show the different adsorption behaviors. Specific additives (—OH, —O—and—CON—) play a major role in determining the viscoelastic of absorption layer.