The RSA public and private keys of biometric certificate can be generated from biometric key which can be seen as random numbers.However, the size of biometric key is shorter than the RSA public and private keys. To overcome this limitation, a biometric certificate key generation algorithm is proposed. In this method, the biometric primes is generated by the combination of symmetric key encryption algorithm and prime generation algorithm, in addition, the hashing algorithm is used to ensure the feasibility of the biometric primes. The generated biometric primes are safe and usable so that they can be applied to generate the RSA public and private keys of biometric certificate. Experimental results using VC6.0 and MIRACL show that the proposed method not only is feasible, but also has practical application value.