The biological stability is closely associated with the safety of drinking water and can be controlled effectively by prohibiting bacterial regrowth. The effects of three main nutrients (organic carbon, phosphorus, nitrogen) and chlorine on bacterial regrowth in drinking water were investigated using BRP method in lab-scale experiments. The critical values of carbon, nitrogen and phosphorus promoting bacterial growth were 250, 7 and 3 μg/L respectively, while the yield values for heterotrophic bacteria of the three nutrients were 2.35×10⁶(R²=0.99, p < 0.05), 9.76×10⁷ (R²=0.91, p < 0.05) and 1.76×10⁸ CFU/μg (R²=0.91, p < 0.05) during the absence of only one kind of nutrient. When two kinds of nutrients were in deficiency, nutrient which was relatively low would be the main BRP limiting factor and decrease the supporting ability of the other nutrients on bacterial regrowth. Moreover, BRP decrease with the increasing amount of free chlorine in drinking water. When the concentration of free chlorine reached 0.5 mg/L, the growth of bacteria was inhibited obviously. In other words, free chlorine weakened the ability of nutrients supporting bacterial regrowth.