Taking an 80-meter-span singer-layer reticulated dome which has been constructed in the Shenbei New District as an example, the natural frequency characteristics and dynamic response of the singer-layer reticulated dome under different peak seismic loads were analyzed with the FE software ANSYS. Based on the data of the numerical analysis and the test of the joints, the bending performance of bolt-ball joints were obtained and introduced into the seismic response analysis considering the joint stiffness. Different structural models were established to investigate the effect of the structure model changes, including strengthening the local members and joints and installing maintenance materials on the surface of the structure. The results show that the constructed 80-meter-span singer-layer reticulated dome has a good seismic performance, but the local hole on the structure is the obvious weak area. It is suggested that the welded ball joints should be used around the hole, and the members around the hole should be strengthened. The seismic performance of the structure will be reduced by installing maintenance materials on the surface, and the maximum displacement of the reticulated dome increases by 140.1%, and the maximum internal force increases by 77.8% under the rare earthquake action. A divergence trend of the curve can be found in the time-displacement curve of the structure, showing the possibility of a whole collapse of the structure.