A new atrazine-degrading strain named ZXY-1 was isolated in the present study. ZXY-1 was identified as Pseudomonas based on the physio-biochemical characteristics and 16s rRNA gene sequence analysis. The newly isolated strain had a strong ability to biodegrade atrazine of 100 mg/L completely within 11 h, with a degrading rate up to 9.09 mg/(L·h). The optimal conditions for atrazine degradation were captured: temperature 30 ℃, pH 8.0, velocity of shaker 150 r/min and inoculums 3%. Atrazine biodegradation, under the optimum conditions, fitted the first-order kinetic equation when the initial atrazine concentration ranged from 20 mg/L to 80 mg/L, and the zero-order kinetic equation when the initial atrazine concentration ranged from 100 mg/L to 250 mg/L. The kinetic analysis indicated that the strain ZXY-1 cell growth perfectly suited Haldane model with μ_max, K_s and K_i of 0.696 h^-1, 98.55 and 142.6 mg/L, respectively. Overall, this study provided an efficient bacterium and approach that might serve as a viable bioremediation solution to treat the atrazine wastewater.