To investigate ruin characteristic and rules of survival space in the RC frame supported masonry structures after earthquake, seismic investigation was carried out and FEM analysis and shaking table tests were conducted. The three main collapse modes were summarized (the collapses of frame floor, transfer floor and overall structure) based on seismic investigation. The result shows that collapse of frame floor is the most common type. The connection mode and the loose mode are the two main ruin modes. The connection mode owns more survival space and is difficult for rescue, and more survival space is on the reverse direction of collapse. The top floor of frame structure has more survival spaces than the bottom floor. The loose mode owns little survival space and is easier for rescue than the connection mode. There is a certain degree of survival space on the less wall position near the beam-column, and under the beam, which lies in the reverse direction of collapse. The FEM collapse analysis of frame floor was conducted, which was based on ANSYS-LSDYNA, and the results were verified by comparing with the results of seismic investigation and shaking table tests. It shows that there is more survival space on the position of reverse direction of collapse with fewer walls. It contributes to locate the rescue position quickly after earthquake and provides important advises for evacuation.