To identify the damage member location for space steel structures after earthquake, a single-layer reticulated shell structure test model was designed with a single damaged member to study the location identification method. In consideration of the characteristics of spatial steel structure, such as multi-degree of freedom and dense modal, the acceleration responses of two monitoring stages(i.e., before and after structural damage)were used to establish the damage identification index based on wavelet packet energy analysis. The numerical analysis data of 17 different damage locations of members were established as the damage sample base using the finite element software ANSYS. The acceleration response data of the test structure with single member damage were processed and clustered with the damage sample base data to identify possible damage location of the actual structural members. The method can quickly identify the possible damage location of the damaged member in the actual structure and the experimental results validated the feasibility and effectiveness of the proposed method, which provides theoretical and experimental supports for engineering application.