To study the bearing capacity of high strength bolt connection, static tensile tests on longitudinal seam of corrugated steel plate (CSPs) specimens were carried out by considering hot-dip galvanized and non-galvanized steel plates with different thicknesses, and flat plate specimens were designed under the same condition for comparison. The failure mode, the ultimate load and slip load of the longitudinal seam, and the limit and slip displacement of the specimens were analyzed. Results reveal that hot-dip galvanizing almost did not affect the ultimate load. When bearing failure at bolt hole occurred, the ultimate load of the CSP connections was about 12% to 20% higher than that of the flat plate specimens, while there was little difference in the limit displacement between them. When bolt shearing failure occurred, the ultimate displacement of the flat plate connections was about 1.3 to 1.7 times of the CSP ones, but their ultimate bearing capacities were similar. Based on the comparison between the standard calculation result and the test result, it suggests that the design formulae of JGJ 99—2015 are safe to calculate the bearing capacity of longitudinal seam strength of CSP with high strength bolt connection.