The 24th Winter Olympic Games will be held in China in February 2022. The main venue is located in Chongli, Hebei Province. The outdoor daily average temperature during the competition is estimated to be-17 ℃－-2 ℃. To effectively improve the thermal comfort and viewing experience of outdoor audiences, the analysis of the cold tolerance characteristics of outdoor audiences is very important. In this study, eight subjects (four males and four females) from a university in Beijing participated in the cold tolerance experiment. The thermal response characteristics of different parts of the human body, the maximum permissible time, and the physiological limit thresholds of the subjects under cold exposure conditions (-14 ℃－-16 ℃) were investigated in a commercial cold store in Beijing. Results show that when the subject was exposed to a cold environment of -14 ℃ to -16 ℃ and the thermal insulation of the clothing ensembles was 2.1 clo, the skin temperature of the fingers and toes had the highest rate of decline and the cold sensation was the strongest; the skin temperature of trunk and limbs decreased less, and the cold sensation was weaker. Under the conditions of hand exposure and hands in pockets, the average maximum permissible time was 51 min and 67 min respectively. The key factors affecting the thermal sensation and permissible time of the subjects were the skin temperature of the fingers and toes, with the average tolerance of human body in extreme cold environments and the construction of corresponding local thermal physiological limit thresholds of 11℃ and 16 ℃ respectively, and no significant difference was found between male and female (P＞0.05). This study can provide basic data reference for the research on the coldcomfort environment.