To investigate feasibility and applicability of high-speed railway simply supported beam with a combined seismic isolation system in nine-degree seismic regions, taking the type high-speed railway 32 m simply supported beam as a prototype, and the shaking table tests of a 1/10 scale 3 span bridge model were conducted. Fifteen typical near-field ground motion records were selected as the input excitations, and the vertical+vertical and horizontal+vertical were combined input. The seismic displacement, acceleration, reinforcement strain and other response data of key components of the bridge were collected during the test, the damage of the bridge after each earthquake input condition were observed, and the experimental phenomena and measured data were analyzed comprehensively. The results show that the pier remains elastic under earthquakes, and the relative displacement of pier and beam becomes smaller, the transverse and vertical acceleration of the main beam is controlled within 0.3g and 0.5g, respectively, which ensures the driving safety after the bridge adopts the hyperboloid bearing + metal damping limit device + steel anti-falling beam combined damping. The combined seismic isolation system has played a good effect of damping and isolation under the designed seismic action, and dissipated earthquake energy. The relative displacement of pier and beam is controlled below 20 mm, the pier is slight damage, and its displacement ductility ratio is less than 1.2. The combined seismic isolation system works stably to ensure that the main beam does not suffer from falling earthquake and girder hopping risk under the rare action of earthquake, and the pier is moderate damage, and its displacement ductility ratio is controlled below 2.2, without collapse risk. The combined seismic isolation system has achieved the seismic performance targets of 32 m span simply supported beam of high-speed railway in nine-degree seismic regions.